Phylogenetic Analysis of Cucurbit Chlorotic Yellows Virus from Melon in 2020 in Chungbuk, Korea

Cucurbit chlorotic yellows virus (CCYV) is a plant that causes damage to cucurbit crops such as watermelon and cucumber, transmitted by an insect vector known the whitefly. Since CCYV was first detected on cucumber in Chungbuk 2018, it has been reported other areas including Gyeongsang Korea. In 2020, we performed field surveys of yellowing diseases greenhouses growing melon (Jincheon Eumseong). Reverse transcription-polymerase chain reaction analysis 79 collected samples melon, watermelon, weeds resulted detection 4 samples: Three were singly infected with one mixed CCYV, aphid borne virus, Watermelon mosaic virus. The complete genome sequences four isolates (ES 1–ES 4) determined genetically compared those previously retrieved from GenBank. Phylogenetic analyses RNA 1 2 revealed ES clustered group closely related China. also very low genetic diversity among isolates. general, showed little diversity, regardless host or geographic origins. potential pose serious threat production Further studies are needed examine pathogenicity transmissibility cucurbits watermelon.